This study shows that an alternative host, G. Virulence of five Fno isolates showed strong agreement between G. Fno was phagocytosed and could survive intracellularly, which is consistent with observations in fish. Infection progression was assessed by histopathology (haematoxylin and eosin staining, Gram Twort and immunohistochemistry) and enumeration of bacteria recovered from the larval haemolymph on selective agar.
Larvae were killed by Fno in a dose-dependent manner but the insects could be rescued from lethal doses of bacteria by antibiotic therapy. Here, larvae of Galleria mellonella were assessed for suitability to study Fno virulence. However, studies with native hosts are costly and considerable benefits would derive from access to a practical alternative host. Antibiotics are administered to treat the disease but a better understanding of Fno infection biology will inform improved treatment and prevention measures. orientalis ( Fno) is the etiological agent of francisellosis in cultured warm water fish, such as tilapia.
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